Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Human SCF is an 18.4kDa protein containing 164 amino acid residues.Source:Recombinant protein corresponding to human SCF, expressed in E. coli.Biological Activity:Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is <2.0ng/ml, corresponding to a specific activity of >5x10e5units/mg.Endotoxin:<0.1ng/ug of protein AA Sequence:MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVLPSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKLVNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIFNRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFMLPPVAProtein Content:Verified by UV Spectroscopy and/or SDS-PAGE gel.Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.