Phospholipase D (PLD) is a key player in phospholipid metabolism. PLD hydrolyzes the phosphodiester bond of the glycerophospholipids, resulting in the production of phosphatidic acid and a free headgroup. PLD activity regulates the actin cytoskeleton, vesicle trafficking for secretion and endocytosis, and receptor signaling. Abnormalities in PLD activity and expression have been associated with Alzheimer's disease, stroke, cancer and other brain disorders.Sample Type:Animal tissues: liver, brain, kidney, etc. Cell culture: Adherent or suspension cells.Intended Use:For use in measurement of PLD activity in various tissues/cells, analyzing intracellular signaling and screening of PLD inhibitors.Sensitivity:The assay can detect phospholipase D (PLD) Activity <1mU/ml in various sample types.Test Principle:This Phospholipase D Activity BioAssay Kit provides a simple and sensitive method of measuring PLD activity using colorimetry (OD 570nm). In this assay, PLD cleaves choline from Phosphatidylcholine. The free choline is then oxidized by PLD enzyme mix to generate an intermediate which reacts with PLD Probe to generate color (OD 570nm). Kit Components:PLD Assay Buffer, 1x25ml PLD Probe, in DMSO, 1x200ulPLD Substrate, 1x100ulPLD Enzyme Mix, lyophilized, 1x vialCholine Standard, lyophilized, 1x vialPLD Positive Control, lyophilized, 1x vialStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
产品特点
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保存建议
建议收到Phospholipase D Activity Colorimetric BioAssay Kit (PLD)产品后将其置于-20°C保存。