A metabolite of Methotrexate, which is used as a antineoplastic and antirheumatic.Alternate Name:7-Hydroxy amethopterin, 4-amino-7-hydroxy-10-methylpteroylglutamic acid; N-[4-[[(2,4-Diamino-7,8-dihydro-7-oxo-6-pteridinyl)methyl]methylamino]benzoyl]-L-glutamic acid; NSC 380962CAS Number:5939-37-7Molecular Formula:C₂₀H₂₂N₈O₆Molecular Weight:470.44Purity:~95% (HPLC)Appearance:Supplied as a light yellow to light orange solidMelting Point:>221ºC (dec.)Solubility:Aqueous Basic (Slightly), DMSO (Slightly, Sonicatedy), Methanol (Slightly)Method for Determining Identity:Proton NMR (DMSO-d6, D2O+ NaOD) Spectroscopic and Mass Spectrometric AnalysisTLC Conditions:C18: Acetonitrile: Water: Ammonium Hydroxide=8:2:0.1; Visualized with UV and KMnO4; Single Spot, Rf=0.40Elemental Analysis:Calculated: %C: 51.06, 5H: 4.71, %N: 23.82Storage and Stability:Long-term storage is recommended at -20°C. Stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.