IL-1beta BioAssay ELISA Kit (Human) (Interleukin-1 beta，IL-1 beta，Catabolin，IL1B，IL1F2，IL-1beta，IL1beta)-IL-1beta BioAssay ELISA Kit(Human)(Interleukin-1 beta，IL-1 beta，Catabolin，IL1B，IL1F2，IL-1beta，IL1beta)代理/参数/厂家-细胞分析试剂盒-开云·官方站线上登录平台入口

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细胞分析试剂盒

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中文名称: IL-1beta BioAssay ELISA Kit (Human) (Interleukin-1 beta，IL-1 beta，Catabolin，IL1B，IL1F2，IL-1beta，IL1beta)

英文名字: IL-1beta BioAssay ELISA Kit(Human)(Interleukin-1 beta，IL-1 beta，Catabolin，IL1B，IL1F2，IL-1beta，IL1beta)

供应商: USBiological

产品货号: 360495

产品报价: ￥1/48Tests

产品说明书

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背景资料: USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述: Interleukin 1 （IL-1） includes two distinct proteins，IL-1alpha and IL-1beta，that play central roles in acute and chronic inflammation，both locally and systemically. Human IL-1beta is synthesized as a 269aa，3kD precursor protein （prepro-IL-1beta） that is cleaved by IL-1beta-converting enzyme （ICE） to the 153aa，17kD mature IL-1beta plus a prosegment. Some combination of the mature form，the prosegment and prepro-IL-1beta is released from the cell. IL-1beta is produced primarily by monocytes and macrophages but also by astrocytes，oligodendroglia，adrenal cortical cells，NK cells，endothelial cells，keratinocytes，megakaryocytes，platelets，neurons，neutrophils，osteoblasts，Schwann cells，trophoblasts，T cells，and fibroblasts. The most extensively studied function of IL-1 is initiation of inflammation. Bacterial endotoxin or a variety of non-microbial inflammatory substances induce production of IL-1，which is released into the local environment. IL-1 induces capillary endothelial cells to secrete chemokines （e.g. MCP-1） and to increase expression of cell adhesion molecules （e.g. E-selectin，ICAM-1 and VCAM-1）.Sample Type：Cell culture supernatants，serum，plasma，and tissueIntended Use：The Human IL-1beta BioAssay ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human IL-1beta concentrations in cell culture supernatants，serum，plasma，and tissue.Sensitivity：4pg/mlRange：7.8-500pg/mlSpecificity：Recognizes human IL-1beta. There was no detectable cross reactivity with any of the tested proteins following IL-2,3,4,5,6，8,10,12,TNF,VEGF,TGF and IFN.Sample Volume：100ul/wellTest Principle：This assay employs the Sandwich immunoassay technique. An anti-h IL-1beta monoclonal coating antibody is adsorbed onto microwells. IL-1beta present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h IL-1beta antibody is added and binds to IL-1beta captured by the first antibody. Following incubation unbound Biotinylated anti-h IL-1beta antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h IL-1beta antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of IL-1beta present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-1beta standard dilutions and IL-1beta sample concentration determined.Kit Components：Aluminium pouches with a Microwell Plate： 8×6Standard： 1xVialBiotinylated antibody： 1xVialStreptavidin-HRP： 1xVialAssay Diluent （25ml/Vial）： 1xVialWash Buffer Concentrate 20x （30ml/Vial）： 1xVialTMB Substrate Solution： 1x6mlStop Solution （12ml/Vial）： 1xVialPlate Covers- Adhesive strips： 2xstripsInstruction： 1xStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.

产品特点: 针对IL-1beta BioAssay ELISA Kit (Human) (Interleukin-1 beta，IL-1 beta，Catabolin，IL1B，IL1F2，IL-1beta，IL1beta)该产品的特点优势，欢迎查阅官网提供的产品说明书。

保存建议: 建议收到IL-1beta BioAssay ELISA Kit （Human）（Interleukin-1 beta，IL-1 beta，Catabolin，IL1B，IL1F2，IL-1beta，IL1beta）产品后将其置于4°C至-20°C保存。

其他: Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。