TNF-alpha BioAssay ELISA Kit (Human) (Tumor necrosis factor，Cachectin，TNF-alpha，Tumor necrosis factor ligand superfamily member 2，TNF-a，TNF，TNFA，TNFSF2，TNFalpha，TNF alpha，TNF a，TNFa)-TNF-alpha BioAssay ELISA Kit(Human)(Tumor necrosis factor，Cachectin，TNF-alpha，Tumor necrosis factor ligand superfamily member 2，TNF-a，TNF，TNFA，TNFSF2，TNFalpha，TNF alpha，TNF a，TNFa)代理/参数/厂家-细胞分析试剂盒-开云·官方站线上登录平台入口

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中文名称: TNF-alpha BioAssay ELISA Kit (Human) (Tumor necrosis factor，Cachectin，TNF-alpha，Tumor necrosis factor ligand superfamily member 2，TNF-a，TNF，TNFA，TNFSF2，TNFalpha，TNF alpha，TNF a，TNFa)

英文名字: TNF-alpha BioAssay ELISA Kit(Human)(Tumor necrosis factor，Cachectin，TNF-alpha，Tumor necrosis factor ligand superfamily member 2，TNF-a，TNF，TNFA，TNFSF2，TNFalpha，TNF alpha，TNF a，TNFa)

供应商: USBiological

产品货号: 361329

产品报价: ￥1/48Tests

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背景资料: USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述: The prototype ligand of the TNF superfamily，TNF-alpha/TNFSF1A，is a pleiotropic cytokine that plays a central role in inflammation and apoptosis. It is synthesized as a 26kD，type II transmembrane protein that is 233aa in length. It contains a 30aa cytoplasmic domain，a 26aa transmembrane segment，and a 177aa extracellular region. TNF-alpha is assembled intracellularly to form a transmembrane，non-covalently-linked homotrimeric protein. Human cells known to express TNF-alpha include B cells，colonic columnar epithelial cells，NK and CD3+CD56+ hepatic natural T cells，macrophages，monocytes and monocyte-derived dendritic cells，CD4+ and CD8+ T cells，mast cells，neutrophils，keratinocytes，plasma cells，and adipocytes.Sample Type：Cell culture supernatants，serum，plasma，and tissueIntended Use：The Human TNF-alpha BioAssay ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human TNF-alpha concentrations in cell culture supernatants，serum，plasma，and tissue.Sensitivity：4pg/mlRange：15.625-1000pg/mlSpecificity：Recognizes human IFN-alpha. There was no detectable cross reactivity with any of the tested proteins following IL-1,2,3,4,5,6，8,10,12,VEGF,TGF,TNF and ICAM.Sample Volume：100ul/wellTest Principle：This assay employs the Sandwich immunoassay technique. An anti-h TNF-alpha monoclonal coating antibody is adsorbed onto microwells. TNF-alpha present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h TNF-alpha antibody is added and binds to TNF-alpha captured by the first antibody. Following incubation unbound Biotinylated anti-h TNF-alpha antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h TNF-alpha antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of TNF-alpha present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven TNF-alpha standard dilutions and TNF-alpha sample concentration determined.Kit Components：Aluminium pouches with a Microwell Plate： 8x6Standard： 1xVialBiotinylated antibody： 1xVialStreptavidin-HRP： 1xVialAssay Buffer （25ml/Vial）： 1xVialWash Buffer Concentrate 20x （30ml/Vial）： 1xVialTMB Substrate Solution： 1x6mlStop Solution （12ml/Vial）： 1xVialPlate Covers- Adhesive strips： 2xstripsInstruction： 1xStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.

产品特点: 针对TNF-alpha BioAssay ELISA Kit (Human) (Tumor necrosis factor，Cachectin，TNF-alpha，Tumor necrosis factor ligand superfamily member 2，TNF-a，TNF，TNFA，TNFSF2，TNFalpha，TNF alpha，TNF a，TNFa)该产品的特点优势，欢迎查阅官网提供的产品说明书。

保存建议: 建议收到TNF-alpha BioAssay ELISA Kit （Human）（Tumor necrosis factor，Cachectin，TNF-alpha，Tumor necrosis factor ligand superfamily member 2，TNF-a，TNF，TNFA，TNFSF2，TNFalpha，TNF alpha，TNF a，TNFa）产品后将其置于4°C至-20°C保存。

其他: Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。