Rabbit TrueBlot® Western Blot Kit - 88-8886-31-Rabbit TrueBlot® Western Blot Kit - 88-8886-31代理/参数/厂家-免疫学试剂盒-开云·官方站线上登录平台入口

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中文名称: Rabbit TrueBlot® Western Blot Kit - 88-8886-31

英文名字: Rabbit TrueBlot® Western Blot Kit - 88-8886-31

供应商: Rockland

产品货号: 88-8886-31

产品报价: ￥询价/1Kit

产品说明书

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背景资料: The Rabbit TrueBlot® Western Blot Kit contains the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples using TrueBlot monoclonal secondary antibody in conjunction with your own primary IP antibody and primary (Rabbit IgG) Western blotting antibody (see application note for kit components). Rabbit IgG TrueBlot® is the unique horseradish peroxidase conjugated Anti-Rabbit IgG monoclonal secondary antibody which enables detection of immunoblotted target protein bands, without hindrance by interfering immunoglobulin heavy and light chains from your IP antibody. Use it in place of your conventional HRP Anti-Rabbit IgG immunoblotting secondary antibody. It is easy to generate publication-quality IP/WB data with Rabbit IgG TrueBlot®. Rabbit IgG TrueBlot is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of rabbit IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Rabbit IgG TrueBlot with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/immunoblot applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Rabbit IgG TrueBlot may also be used for detection in immunoblotting assays that do not employ immunoprecipitation.

产品描述: Rabbit IgG TrueBlot® is provided as 1000X solution. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield enough reagent for 20 blots. Rabbit IgG TrueBlot® HRP-conjugated monoclonal secondary antibody reacting with goat IgGs for optimal signal detection in immunoprecipitation/immunoblotting experiments. All recommended dilutions for listed applications are intended as an initial recommendation, specific conditions for each protein and antibody combination should be specifically optimized by the end user. Note that there are three key procedural considerations: 1. Protein A or G should not be used for the immunoprecipitation. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. For immunoprecipitation, Anti-Rat IgG beads, or Anti-Rabbit IgG beads should be used for rat or rabbit immunoprecipitating antibodies, respectively. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot. A sample of Anti-Alpha-Tubulin (MOUSE) Monoclonal Antibody Cat #(200-301-880S), has been provided to be used as a loading control at the user’s discretion. Dilutions are to be optimized by the user. Rabbit TrueBlot Western Blot Kit Components: 1. Rabbit IgG TrueBlot: 50 μL. 18-8816-31; 2. TrueBlot Enhancer Solution: 25 mL; 3. TrueBlot Blocker: 10 g; 4. TrueBlot Assay Buffer: 30 mL, 20X; 5. TrueBlot Substrate A: 12.5 mL; 6. TrueBlot Substrate B: 12.5 mL; 7. Western Blot Incubation Tray. 8. Anti-Alpha-Tubulin (MOUSE) Monoclonal Antibody - 200-301-880S.

产品特点: 该Rabbit TrueBlot® Western Blot Kit - 88-8886-31的详细信息查看Rockland提供的产品说明书。

保存建议: Store Kit at 2-8 °C, except Rabbit TrueBlot® which should be stored at -20 °C. This product is guaranteed for 6 months upon receipt, when handled and stored as instructed.

其他: Rockland成立于1962年，是一家老牌抗体原产企业，其位于美国费城郊区的农场保有近20种实验动物。Rockland公司为生命科学领域提供丰富的、高质量的抗体，包括载脂蛋白、细胞周期/凋亡、细胞因子、细胞信号蛋白、酶类、细胞外基质、转录因子(NFkB)和泛素（Ubiquitin）等识别抗体，应用范围包括Western blot、免疫组化、免疫荧光和ELISA等。另外，Rockland也生产特别齐全的二抗，包括纯化、生物素、HRP/ALP、荧光标记二抗，应用十分广泛。