TrueBlot® Immunoprecipitation and Western Blot Kit for GFP Epitope Tag - 88-8887-215-TrueBlot® Immunoprecipitation and Western Blot Kit for GFP Epitope Tag - 88-8887-215代理/参数/厂家-免疫学试剂盒-开云·官方站线上登录平台入口

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中文名称: TrueBlot® Immunoprecipitation and Western Blot Kit for GFP Epitope Tag - 88-8887-215

英文名字: TrueBlot® Immunoprecipitation and Western Blot Kit for GFP Epitope Tag - 88-8887-215

供应商: Rockland

产品货号: 88-8887-215

产品报价: ￥询价/1Kit

产品说明书

购买方式: 90%产品中国有现货库存。银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868

产品新闻

背景资料: TrueBlot® Immunoprecipitation and Western Blot Kit for GFP Epitope Tag allows for the immunoprecipitation and detection of GFP-tagged recombinant proteins present in cell lysates provided by the user. After protein separation by SDS-PAGE and transfer, the membrane is probed with monoclonal Anti-GFP. Detection of the membrane bound antibody-antigen complex is achieved by the addition of Mouse IgG TrueBlot® ULTRA HRP. The TrueBlot® Immunoprecipitation and Western Blot Kit for GFP Epitope Tag contains the critical supporting reagents, buffers, and substrates for immunoprecipitation and western blot containing a GFP Epitope Tag using TrueBlot secondary antibody immunoblotting reagents in conjunction with Rockland's Anti-GFP (MOUSE) Monoclonal Antibody. Mouse IgG TrueBlot® ULTRA is the unique horseradish peroxidase conjugated Anti-Mouse IgG monoclonal secondary antibody which enables detection of immunoblotted target protein bands, without hindrance from interfering immunoglobulin heavy and light chains from your IP antibody. Use it in place of your conventional HRP Anti-Mouse IgG secondary antibody. It is easy to generate publication-quality IP/WB data with Mouse IgG TrueBlot® ULTRA.

产品描述: Mouse IgG TrueBlot® ULTRA is provided as 1000X solution. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). Mouse IgG TrueBlot® ULTRA is an HRP-conjugated monoclonal secondary antibody reacting with mouse IgGs for optimal signal detection in immunoprecipitation/immunoblotting experiments. All recommended dilutions for listed applications are intended as an initial recommendation, specific conditions for each protein and antibody combination should be specifically optimized by the end user. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries, but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot. TrueBlot® IP and WB Kit for GFP Epitope Components: 1. Mouse IgG TrueBlot ULTRA: 50 μL 18-8817-31; 2. TrueBlot Enhancer Solution: 25 mL; 3. TrueBlot Blocker: 10 g; 4. TrueBlot Assay Buffer: 30 mL 20X; 5. TrueBlot Substrate A: 12.5 mL; 6. TrueBlot Substrate B: 12.5 mL; 7. Anti-Mouse Ig IP Beads: 2.5 mL Binds 0.4 mg Ig/mL beads; 8. Anti-GFP (MOUSE) Monoclonal Antibody: 100 µg; 9. Western Blot Incubation Tray. Special Notes: Upon initial use of the IP beads, we recommend that the vial be inverted several times to get the beads into suspension. We recommend using a large bore pipet to pipet up the liquid for use. For storage of the opened vial of beads, we recommend that the vial cap be sealed with parafilm to help prevent evaporation of the buffer.

产品特点: 该TrueBlot® Immunoprecipitation and Western Blot Kit for GFP Epitope Tag - 88-8887-215的详细信息查看Rockland提供的产品说明书。

保存建议: Store Kit at 2-8 °C, except Mouse TrueBlot® ULTRA and Anti-GFP (MOUSE) Monoclonal Antibody, which should be stored at -20 °C. This product is guaranteed for 6 months upon receipt, when handled and stored as instructed.

其他: Rockland成立于1962年，是一家老牌抗体原产企业，其位于美国费城郊区的农场保有近20种实验动物。Rockland公司为生命科学领域提供丰富的、高质量的抗体，包括载脂蛋白、细胞周期/凋亡、细胞因子、细胞信号蛋白、酶类、细胞外基质、转录因子(NFkB)和泛素（Ubiquitin）等识别抗体，应用范围包括Western blot、免疫组化、免疫荧光和ELISA等。另外，Rockland也生产特别齐全的二抗，包括纯化、生物素、HRP/ALP、荧光标记二抗，应用十分广泛。