ANGPTL3, Mouse, BioAssay ELISA Kit (Angiopoietin-related Protein 3, Angiopoietin-like Protein 3)-ANGPTL3, Mouse, BioAssay ELISA Kit (Angiopoietin-related Protein 3, Angiopoietin-like Protein 3)代理/参数/厂家-细胞分析试剂盒-开云·官方站线上登录平台入口

PRODUCT CENTER

细胞分析试剂盒

开云·官方站线上登录平台入口_开云(中国)

当前位置：开云·官方站线上登录平台入口 > 开云·官方站线上登录平台入口_开云(中国) > 细胞生物学产品 > 细胞分析试剂盒

中文名称: ANGPTL3, Mouse, BioAssay ELISA Kit (Angiopoietin-related Protein 3, Angiopoietin-like Protein 3)

英文名字: ANGPTL3, Mouse, BioAssay ELISA Kit (Angiopoietin-related Protein 3, Angiopoietin-like Protein 3)

供应商: USBiological

产品货号: A2293-48R

产品报价: ￥1/100Tests

产品说明书

购买方式: 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868

产品新闻

背景资料: USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述: The angiopoietins are a family of growth factors that are specific for vascular endothelium. The full-length cDNA encoding angiopoietin-like protein 3 (ANGPTL3) from a human fetal liver/spleen cDNA library has 460aa and the characteristic structure of angiopoietins: a signal peptide, an extended helical domain predicted to form dimeric or trimeric coiled-coils, a short linker peptide, and a globular fibrinogen-like domain (FLD). Human ANGPTL3 shares 76% aa sequence identity with mouse Angptl3. Northern blot analysis of human tissues showed a preferential expression of 4 ANGPTL3 transcripts being 4.5, 3.0, 2.8, and 1.7kb in liver. ANGPTL3 can induce angiogenesis in the rat corneal assay. The FLD alone was sufficient to induce endothelial cell adhesion and in vivo angiogenesis. Microarray analysis showed that mouse hematopoietic stem cell (HSC)-supportive fetal liver CD3-positive cells expressed Angptl2 and Angptl3. The ANGPTL3 ELISA Kit is to be used for the in vitro quantitative determination of mouse/ rat ANGPTL3 in biological fluids. This assay is a sandwich ELISA for quantitative determination of mouse or rat ANGPTL3 in biological fluids. A polyclonal antibody specific for ANGPTL3 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, ANGPTL3 is recognized by the addition of a biotinylated polyclonal antibody specific for ANGPTL3 (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of ANGPTL3 in the samples. The assay range is 0.016-1ng/ml ANGPTL3/ml. The lowest level of ANGPTL3 that can be detected by this assay is 15pg/ml.Sample Type:Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids.Intended Use:For the in vitro quantitative determination of mouse/ rat ANGPTL3 in biological fluids.Sensitivity:15pg/ml Range:0.016-1ng/ml ANGPTL3/mlSpecificity:MammalianTest Principle:This assay is a sandwich ELISA for quantitative determination of mouse or rat ANGPTL3 in biological fluids. A polyclonal antibody specific for ANGPTL3 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, ANGPTL3 is recognized by the addition of a biotinylated polyclonal antibody specific for ANGPTL3 (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of ANGPTL3 in the samples.Kit Components:Pre-coated Microtiter Plate: 12x8 well stripsWash Buffer (10X): 1x50mlDiluent (5X):1x50mlDetection Antibody: 1x12mlDetector 100X (HRP labeled Streptavidin): 1x150ulMouse ANGPTL3 Standard (lyophilized): 1 vialMouse ANGPTL3 QC Sample (lyophilized): 1 vialSubstrate Solution (TMB): 1x6mlStop Solution: 1x6mlPlate Sealers (plastic film): 3 eachStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

产品特点: 针对ANGPTL3, Mouse, BioAssay ELISA Kit (Angiopoietin-related Protein 3, Angiopoietin-like Protein 3)该产品的特点优势，欢迎查阅官网提供的产品说明书。

保存建议: 建议收到ANGPTL3, Mouse, BioAssay ELISA Kit (Angiopoietin-related Protein 3, Angiopoietin-like Protein 3)产品后将其置于4°C保存。

其他: Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。