Rat ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) ELISA Kit
供应商
Biomatik
产品货号
EKU02629
产品报价
¥询价/96T
产品说明书
购买方式
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产品新闻
背景资料
This assay has high sensitivity and excellent specificity for detection of ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2). No significant cross-reactivity or interference between ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) and analogues was observed.
产品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Voltage Dependent Anion Channel Protein 1 (VDAC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Voltage Dependent Anion Channel Protein 1 (VDAC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Voltage Dependent Anion Channel Protein 1 (VDAC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Voltage Dependent Anion Channel Protein 1 (VDAC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该大鼠ATPase,H+转运,溶酶体辅助蛋白2(ATP6AP2)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.