Human Glycogen Phosphorylase, Muscle (PYGM) ELISA Kit
供应商
Biomatik
产品货号
EKU04518
产品报价
¥询价/96T
产品说明书
购买方式
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产品新闻
背景资料
This assay has high sensitivity and excellent specificity for detection of Glycogen Phosphorylase, Muscle (PYGM). No significant cross-reactivity or interference between Glycogen Phosphorylase, Muscle (PYGM) and analogues was observed.
产品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该人糖原磷酸化酶,肌肉(PYGM)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.