中文名称

【GST他的】重组谷胱甘肽S-转移酶，他的标签

英文名字

Recombinant E.Coli Glutathione S-Transferase His Tag

供应商

ProSpec

产品货号

ENZ-451

产品报价

￥询价/10ug   ￥1mg   ￥50ug

产品说明书

购买方式

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产品新闻

背景资料

Antioxidant enzyme Glutathione S- Transferase（GST) is thought to do the primary cellular defense mechanism against reactive oxygen species. GST reduces lipid hydroperoxides through its Se-independent glutathione peroxidase activity. The enzyme also detoxifies lipid peroxidation end products such as 4-hydroxynonenal（4-HNE).
The soluble GST is a 26 kDa protein which occurs as a dimer in all aerobic organisms. Each monomer has two domains， one that binds GSH and is an /-structure similar to thioredoxin and the other， all helical， that binds the hydrophobic substrate. The GST -fusion protein expression system is a widely used recombinant protein expression system that allows a peptide or a regulatory protein domain to be expressed as a fusion to the C-terminus of Schistosoma japonicum GST. Fusion proteins also possess GST -enzymatic activity and can undergo dimerization similar to in vivo. The fusion protein can be purified via GST -affinity column chromatography. In most cases， the desired peptides or domains are removed from GST by applying a specific protease that recognizes and cleaves the linker between the protein domain and GST. The technique has been widely used to generate different kinds of proteins for crystallization， molecular immunology studies， the production of vaccines and studies involving protein-protein and protein-DNA interactions.

序列

MGSSHHHHHH SSGLVPRGSH MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMAIIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALDVVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPR.

来源宿主

大肠杆菌中重组表达

溶解建议

提供的谷胱甘肽S-转移酶，His标签（GST His）产品为过滤灭菌的透明溶液；其中谷胱甘肽S-转移酶，His标签（GST His）产品溶解于PBS pH 7.4，10%甘油溶液中.

保存建议

谷胱甘肽S-转移酶，His标签（GST His）溶液可以在-20℃到-80℃温度下冻存用于长期保存，保质期为12月.建议分装以避免反复冻融.

其他

ProSpec 是一家全球著名细胞因子蛋白生产和研发的公司，具有20多年的生产经验，专注于蛋白（重组及合成）生产研发。我们的核心产品包括：干扰素、白介素、骨蛋白、肿瘤坏死因子、成纤维生长因子、瘦素、干细胞因子、催乳素、表皮生长因子、胰岛素样生长因子、神经生长因子、集落刺激因子、激素、酶、病毒抗原以及各种重组蛋白等。 领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。

注意

该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。

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