Interleukin 11(IL-11) is a protein that in humans is encoded by the IL11 gene. The protein encoded by this gene is a member of the gp130 family of cytokines. This gene is mapped to 19q13.42. It is a key regulator of multiple events in hematopoiesis, most notably the stimulation of megakaryocyte maturation. IL-11 has been demonstrated to improve platelet recovery after chemotherapy-induced thrombocytopenia, induce acute phase proteins, modulate antigen-antibody responses, participate in the regulation of bone cell proliferation and differentiation and could be use as a therapeutic for osteoporosis. What's more, IL-11 can stimulate the growth of certain lymphocytes and it has functions in many other tissues, including the brain, gut, testis and bone. In transgenic mice, overexpression of the human IL11 gene resulted in the stimulation of bone formation to increase cortical thickness and strength of long bones.
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Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse IL-11 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each mouse IL-11 standard solution and each sample be measured in duplicate.
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.