EpiQuik通用三甲基化组蛋白H4-K20定量分析试剂盒(比色法)专门为检测总体组蛋白H4-K20的三甲基化而设计.检测实验中,而第20位赖氨酸被三甲基化的组蛋白H4结合到表面吸附了抗三甲基化组蛋白H4-K20抗体的微孔上.被捕获的三甲基组蛋白H4-K20被结合了一种显色剂的特异性抗体识别.三甲基化组蛋白H4-K20的量与吸光度成正比,其总量可以通过对比对照组来测定.该通用三甲基化组蛋白H4-K20定量试剂盒(比色法)内主要产品组份包括:C1 (10X wash buffer)
C2 (antibody buffer)
C3 (detecting antibody, 1 mg/ml)
C4 (color developer)
C5 (stop solution)
Standard control (100 ug/ml)
Signal report solution
Signal enhancer
8 well sample strips (with frame)
8 well standard control strips
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已发表的部分文献: 标题:Histone Methylation Marks on Circulating Nucleosomes as Novel Blood-Based Biomarker in Colorectal Cancer作者:Gezer U, Ebru E. Yörüker 1, Metin Keskin 2, Cemil Burak Kulle 2, Yoganiranjana Dharuman 3 and Stefan Holdenrieder 3;杂志名称:Int J Mol Sci 标题:Plasma Histone H4 and H4K20 Trimethylation Levels Differ Between Colon Cancer and Precancerous Polyps.作者:Özgür E, Keskin M, Yörüker EE, Holdenrieder S, Gezer U. ;杂志名称:In Vivo 标题:Epigenetic regulations in alternative telomere lengthening: Understanding the mechanistic insight in arsenic-induced skin cancer patients.作者:Bhattacharjee P, Das A, Giri AK, Bhattacharjee P;杂志名称:Sci Total Environ 标题:Perinatal inflammation alters histone 3 and histone 4 methylation patterns: Effects of MiR-29b supplementation.作者:Sugar SS, Heyob KM, Cheng X, Lee RJ, Rogers LK;杂志名称:Redox Biol 标题:Advances in liquid biopsy in neuroblastoma.作者:Zhuo Z, Lin L, Miao L, Li M, He J;杂志名称:Fundam Res Epigentek 公司是全球领先的表观遗传学相关研究的技术创新者和产品开发供应商。开发了超过700多个专利产品,为表观遗传学方面的研究和新药研发提供全面系统的解决方案,涵盖样品制备、DNA/RNA甲基化(5-mc, 5-hmc, 5-fc, m6A)/DNMT分析、ChIP、组蛋白甲基化/乙酰化/去乙酰化/磷酸化/SUMO-等领域,提供小分子抑制剂、蛋白质、抗体、以及分析试剂盒产品。