通过在裂解缓冲液(IOmM HEPES pH7.9、1.5mM MgCl2、IOmM KCl、ImM乙二胺四乙酸、10%甘油、1%NP-40和蛋白酶抑制剂的混合物)中均质化来制备肝肿瘤组织裂解物。通过离心除去组织和细胞碎片。将产物在含有50 mM DTT的lx SDS样品缓冲液(50 mM Tris-HCl pH 6.8,12.5%甘油,1%十二烷基硫酸钠,0.01%溴酚蓝)中煮沸5分钟。
产品描述
肝肿瘤裂解物以含有50mM DTT的SDS样品缓冲液提供。
产品形式
该Liver Tumor Lysate 产品,产品形式为液体
保存建议
ProSci品牌厂家建议该Liver Tumor Lysate 产品,Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.