TPH2 Peptide is used for blocking the activity of TPH2 antibody (catalog number 42-340). The TPH2 antibody ships at 0.5 mg/mL and the TPH2 Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μL water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
序列
RGFSLDSAVPEEHQ
来源宿主
人
溶解建议
艾美捷温馨提示:开盖前,请先离心。厂家建议您,按照该货号产品的说明书中要求的方式进行溶解和使用。
保存建议
Store at -20˚C. When reconstituted minimize freezing and thawing.