Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
应用类型
E, WB
免疫原
Biotin-GAPDH antibody was raised against a 16 amino acid peptide near the carboxy terminus of human GAPDH.
来源宿主
小鼠
反应性
人, 小鼠, 大鼠, 兔
保存建议
Biotin-GAPDH antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.