BNP is mainly expressed by ventricular myocardium in response to volume overload and increased filling pressure. The BNP gene encodes a 134-amino acid preproBNP precursor, which is converted to 108-amino acid proBNP by the proteolytic cleavage of a 26-amino acid signal peptide (26). ProBNP is further cleaved into the the N-terminal fragment of proBNP (NTproBNP, 1-76) and the C-terminal region biologically active BNP hormone (77-108) (30) also named BNP-32 and additional C-terminal fragments (cf. //www.uniprot.org/uniprot/P16860#PRO_0000001532; (Kambayashi et al., 1990; Nakagawa et al., 2019; Sudoh et al., 1989).
产品描述
Method
Sandwich ELISA, HRP/TMB, 12×8-well detachable strips
Sample type
Serum, EDTA plasma (and saliva -independently validated)
Sample volume
50 µl / well
Assay time
3 h / 30 min
Sensitivity
3.0 pmol/l (= 25.4 pg/ml)
Standard range
0 – 640 pmol/l (= 0 – 5,424 pg/ml)
Conversion factor
1 pmol/l = 8.475 pg/ml (MW: 8.475 kDa)
Refers to NT-proBNP (1-76) that is detected by this ELISA.
Precision
In-between-run (n=8): ≤ 7 % CV
Within-run (n=3): ≤ 4% CV
Cross-reactivity
The assay works with human samples only.